IP Indian Journal of Anatomy and Surgery of Head, Neck and Brain
Official Publication of Khyati Education And Research Foundation
Official Publication of Khyati Education And Research Foundation
Print ISSN: 2581-5210
Online ISSN: 2581-5229
CODEN : IIJAAL
IP Indian Journal of Anatomy and Surgery of Head, Neck and Brain (IJASHNB) open access, peer-reviewed quarterly journal publishing since 2015 and is published under the Khyati Education and Research Foundation (KERF), is registered as a non-profit society (under the society registration act, 1860), Government of India with the vision of various accredited vocational courses in healthcare, education, paramedical, yoga, publication, teaching and research activity, with the aim of faster and better dissemination of knowledge, we will be publishing more...Original Article
Author Details :
Volume : 6, Issue : 3, Year : 2020
Article Page : 92-99
https://doi.org/10.18231/j.ijashnb.2020.025
Abstract
Introduction: The Cyclin D1 gene (CCND1) located on chromosome 11q13 is a positive regulator of the cell cycle. It encodes a nuclear protein that plays an important role in the tumorigenesis of human cancers.
Aim: The aim of the study is to evaluate numerical aberration of Cyclin D1 gene by using Fluorescence in Situ Hybridization (FISH) in Oral Squamous Cell Carcinoma (OSCC).
Materials and Methods: Formalin-fixed paraffin embedded tumor section obtained from histological confirmed 50 OSCC patients from Department of Oncology, ENT and Pathology, S.M.S. Medical College, Jaipur. The FISH technique was used to detect the numerical aberrations of Cyclin D1 using the Vysis protocol.
Results: The CCND1 numerical aberration was found positive in 18(36.0%) of 50 patients of OSCCs. Low level amplification was found in 9(11.3%), Cluster amplification 6(7.5%), polysomy 2(2.5%) and deletion of Cyclin D1 1(1.3%) respectively. There was not statistically significant association of histopathological differentiation, site of carcinoma, lymph node metastasis with gender. A significant association (P=0.004) was present between stage of carcinoma and types of numerical aberration of Cyclin D1 gene. Cyclin D1 aberration showed a significant association with lymph node metastasis (P=0.038). We have found no significant association between risk factors (tabacco chewing, smoking and alcohol consumption) to aberration of Cyclin D1 gene.
Conclusions: Analysis of the CCND1 numerical aberration using FISH on paraffin embedded tumor section may be a useful and practical method for predicting aggressive tumors, recurrence and clinical outcome in patients with OSCCs.
Keywords: Amplification, Cyclin D1, FISH, Numerical Aberration.
How to cite : Yadav S, Saxena D, Leela V , Singh J, Kumar B, A study to detect numerical aberrations of cyclin D1 gene using Fluorescence in situ hybridization technique among patients of oral squamous cell carcinoma. IP Indian J Anat Surg Head Neck Brain 2020;6(3):92-99
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